RESUMO
BACKGROUND: With the increasing popularity of plant protein-based diets, soy proteins are favored as the most important source of plant protein worldwide. However, potential food allergy risks limit their use in the food industry. This work aims to reveal the mechanism of ß-conglycinin-induced food allergy, and to explore the regulatory mechanism of heat treatment and high hydrostatic pressure (HHP) treatment in a BALB/c mouse model. RESULTS: Our results showed that oral administration of ß-conglycinin induced severe allergic symptoms in BALB/c mice, but these symptoms were effectively alleviated through heat treatment and HHP treatment. Moreover, ß-conglycinin stimulated lymphocyte proliferation and differentiation; a large number of cytokines interleukin (IL)-4, IL-5, IL-10, IL-12 and IL-13 were released and interferon γ secretion was inhibited, which disrupted the Th1/Th2 immune balance and promoted the differentiation and proliferation of naive T cells into Th2-type cells. CONCLUSION: Heat/non-heat treatment altered the conformation of soybean protein, which significantly reduced allergic reactions in mice. This regulatory mechanism may be associated with Th1/Th2 immune balance. Our results provide data support for understanding the changes in allergenicity of soybean protein within the food industry. © 2024 Society of Chemical Industry.
RESUMO
Toxic sodium cyanate is always present in cyanide-contaminated waste. A new technology for the efficient decomposition of toxic sodium cyanate by hematite was first proposed in this study. The decomposition of sodium cyanate under various atmospheres has been studied. Studies show that sodium cyanate decomposes above 782 °C in Ar and above 627 °C in air. Sodium cyanate does not decompose even roasted at 400 °C for 120 min in air. Hematite does not promote the decomposition of sodium cyanate in Ar. However, almost all sodium cyanate decomposes efficiently at 400 °C and the mass ration of hematite to sodium cyanate of 1:1 for 30 min in air or oxygen atmosphere. The increased mass ratio of hematite to sodium cyanate and roasting temperature can both favor the efficient decomposition of sodium cyanate. The efficient decomposition of sodium cyanate occurs within 30 min, and it is almost stagnant with the prolongation of roasting time. When roasted in air or oxygen in the presence of hematite, sodium cyanate decomposes to Na2CO3, CO2 and N2 and a small amount of NaNO3 and NOx. The optimal efficient decomposition of sodium cyanate is to roast above 400 °C for 30 min in air or O2 at a mass ration of hematite to sodium cyanate greater than 1:1.
